Publicações relacionadas à zika

In:

Memórias do Instituto Oswaldo Cruz

Authors:

Camila Zanluca, Vanessa Campos Andrade de Melo, Ana Luiza Pamplona Mosimann, Glauco Igor Viana dos Santos, Claudia Nunes Duarte dos Santos, Kleber Luz

In the early 2015, several cases of patients presenting symptoms of mild fever, rash, conjunctivitis and arthralgia were reported in the northeastern Brazil. Although all patients lived in a dengue endemic area, molecular and serological diagnosis for dengue resulted negative. Chikungunya virus infection was also discarded. Subsequently, Zika virus (ZIKV) was detected by reverse transcription-polymerase chain reaction from the sera of eight patients and the result was confirmed by DNA sequencing. Phylogenetic analysis suggests that the ZIKV identified belongs to the Asian clade. This is the first report of ZIKV infection in Brazil.

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In:

Journal of Clinical Virology

Authors:

Guilherme A. Calvet, Ana Maria B. Filippis, Marcos Cesar L. Mendonc, Patricia C. Sequeirab, Andre M. Siqueiraa, Valdilea G. Velosoc, Rita M. Nogueira, Patrícia Brasil

Abstract:

Since May 2015, Brazil’s Ministry of Health has reported autochthonous transmission of Zika virus (ZIKV) in some states of the country. Simultaneous circulation of Dengue, Chikungunya and ZIKV in the country hinder both the diagnosis and the therapeutic approach of patients seeking care with acute febrile illnesses especially in patients with comorbidities. The association between HIV infection and endemic diseases has been described especially in tropical regions with varying levels of complications, although there has been no report of ZIKV in HIV-infected patients. We report the first autochthonous case of laboratory confirmed ZIKV infection in a HIV-infected patient in Rio de Janeiro, Brazil. He evolved with only mild symptoms and recovered well without major laboratory abnormalities. Phylogenetic analysis of the ZIKV detected in the patient sera clustered within the Asian clade. To the best of our knowledge, this is the first time that Zika virus co-infection is reported in a HIV-infected patient.

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In:

Neglected Tropical Diseases

Authors:

Oumar Faye, Caio C. M. Freire, Atila Iamarino, Ousmane Faye, Juliana Velasco C. de Oliveira, Mawlouth Diallo, Paolo M. A. Zanotto, Amadou Alpha Sall

Zika virus (ZIKV) is a mosquito-borne flavivirus, a member of the Spondweni serocomplex, whose natural transmission cycle involves mainly vectors from the Aedes genus (A. furcifer, A. taylori, A. luteocephalus and A. africanus) and monkeys [1], while humans are occasional hosts. Clinical pictures range from asymptomatic cases to an influenza-like syndrome associated to fever, headache, malaise and cutaneous rash [2,3]. Likewise, direct contact is also considered a potential route of transmission among humans, probably during sexual intercourse [4]. The first isolation of ZIKV was in 1947 from the blood of a sentinel Rhesus monkey No. 766, stationed in the Zika forest, near the Lake Victoria in Uganda, and in 1948 ZIKV was also isolated in the same forest from a pool of A. africanus mosquitoes [5]. Thereafter, serological and entomological data indicated ZIKV infections in the African continent in Nigeria in 1971 and 1975 [6], Sierra Leone in 1972 [7], Gabon in 1975 [8], Uganda in 1969 and 1970 [9], Central African Republic in 1979 [10], Senegal from 1988 to 1991 [11] and Coˆte d’Ivoire in 1999 [12]. Recently, ZIKV was detected in Senegal in 2011 and 2012 (unpublished data). In addition, ZIKV infections in Asia were reported in Pakistan [13], Malaysia [14], Indonesia in 1977 and 1978 [15], Micronesia in 2007 [16,17] and Cambodia in 2010 [18]. Although ZIKV was repeatedly isolated, only 14 human cases were reported before April 2007, when a Zika fever (ZF) epidemic occurred in Yap island in Micronesia, where 49 confirmed cases and 73% of the residents older than 3 years provided serologic evidence for recent ZIKV infection [16]. This outbreak showcased the potential of ZF as an emerging disease, which could be misdiagnosed as dengue fever, as happened during the beginning of the Micronesian outbreak [16,17].

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In:

Genome Announcements

Authors:

Mariana Sequetin Cunha, Danillo Lucas Alves Esposito, Iray Maria Rocco,a Adriana Yurika Maeda, Fernanda Gisele Silva Vasami, Juliana Silva Nogueira, Renato Pereira de Souza, Akemi Suzuki,a Marcelo Addas-Carvalho, Maria de Lourdes Barjas-Castro, Mariângela Ribeiro Resende, Raquel Silveira Bello Stucchi, Ilka de Fátima Santana Ferreira Boin, Gizelda Katz, Rodrigo Nogueira Angerami, Benedito Antonio Lopes da Fonseca

We report here the genome sequence of Zika virus, strain ZikaSPH2015, containing all structural and nonstructural proteins flanked by the 5= and 3= untranslated region. It was isolated in São Paulo state, Brazil, in 2015, from a patient who received a blood transfusion from an asymptomatic donor at the time of donation.

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In: 

Virology Journal

Authors:

Oumar Faye, Ousmane Faye, Diawo Diallo, Mawlouth Diallo, Manfred Weidmann and Amadou Alpha Sall

Abstract

Background: Zika virus (ZIKV), a mosquito borne flavivirus is a pathogen affecting humans in Asia and Africa. ZIKV infection diagnosis relies on serology–which is challenging due to cross-reactions with other flaviviruses and/or absence or low titer of IgM and IgG antibodies at early phase of infection- virus isolation, which is labor intensive, time consuming and requires appropriate containment. Therefore, real-time RT-PCR (rRT-PCR) is an appealing option as a rapid, sensitive and specific method for detection of ZIKV in the early stage of infection. So far, only one rRT-PCR assay has been described in the context of the outbreak in Micronesia in 2007. In this study, we described a one step rRT-PCR for ZIKV which can detect a wider genetic diversity of ZIKV isolates from Asia and Africa.

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